SIDT1 Polyclonal antibody, PBS Only

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• SIDT1 Polyclonal antibody, PBS Only (55352-1-PBS)

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  WB analysis using 55352-1-AP (same clone as 55352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55352-1-AP (SIDT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55352-1-AP (same clone as 55352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55352-1-AP (same clone as 55352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 55352-1-AP (same clone as 55352-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SIDT1 antibody (55352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

SIDT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55352-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SID1 transmembrane family member 1, SID1, SID 1

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Product Information

55352-1-PBS targets SIDT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SID1 transmembrane family, member 1
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: NM_017699
• Gene Symbol: SIDT1
• Gene ID (NCBI): 54847
• RRID: AB_11182721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXL6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIDT1, also known as a human orthologue of SID-1, belongs to the SID family which is a highly conserved group of transmembrane channel-like proteins. As a transmembrane channel for intercellular communication, SIDT1 was found to facilitate small interfering RNA (siRNA) or miRNA uptake and also involved in cholesterol transport in human systems. SIDT1 mRNA and protein were mainly detected in the stomach and large intestine (including the cecum, colon and rectum) but not in the small intestine (especially the duodenum). Notably, SIDT1 was mainly located in gastric surface mucous cells (pit cells) but barely expressed in the parietal or chief cells. The SIDT1 gene encodes an 827 amino acid protein with a calculated molecular mass of 94 kDa. (PMID: 31632087, PMID: 32801357, PMID: 22174421)