Tested Applications
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13454-1-AP targets Siglec-6 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4281 Product name: Recombinant human SIGLEC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-200 aa of BC035359 Sequence: QERRFQLEGPESLTVQEGLCVLVPCRLPTTLPASYYGYGYWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRDNAAYFFRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGTPPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHST Predict reactive species |
| Full Name | sialic acid binding Ig-like lectin 6 |
| Calculated Molecular Weight | 426 aa, 47 kDa |
| GenBank Accession Number | BC035359 |
| Gene Symbol | SIGLEC6 |
| Gene ID (NCBI) | 946 |
| RRID | AB_2189287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Siglec-6, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Siglec-6 antibody 13454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Siglec-6 Polyclonal antibody (Cat# 13454-1-AP) has 1 total citation. It was used in Western blot in a 2024 study published in Journal of Advanced Research (IF 17.1), investigating ST6GALNAC1-mediated sialylation in uterine endometrial epithelium and its role in facilitating epithelium-embryo attachment. The research falls within reproductive biology and glycobiology, focusing on sialic acid–mediated signaling at the maternal–embryonic interface.
| Species | Application | Title |
|---|---|---|
J Adv Res ST6GALNAC1-mediated sialylation in uterine endometrial epithelium facilitates the epithelium-embryo attachment |
Reviews
What customers say
The antibody performed well in immunofluorescence on FFPE prostate tissue using Tris-EDTA antigen retrieval at a 1:25 dilution. The reviewer rated it 4 out of 5 stars, confirming reliable staining results for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emma (Verified Customer) (02-22-2022) | Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).
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