Siglec-7/CD328 Polyclonal antibody
Siglec-7/CD328 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13939-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue, human liver tissue, U-937 cells, mouse placenta tissue |
Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
13939-1-AP targets Siglec-7/CD328 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Siglec-7/CD328 fusion protein Ag4220 |
Full Name | sialic acid binding Ig-like lectin 7 |
Calculated Molecular Weight | 467 aa, 51 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC028150 |
Gene Symbol | Siglec-7 |
Gene ID (NCBI) | 27036 |
RRID | AB_10919158 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).
Protocols
Product Specific Protocols | |
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WB protocol for Siglec-7/CD328 antibody 13939-1-AP | Download protocol |
IHC protocol for Siglec-7/CD328 antibody 13939-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Lett Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer. | ||
Commun Biol ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.
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