Siglec-7/CD328 Polyclonal antibody

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  WB analysis using 13939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13939-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13939-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13939-1-AP (Siglec-7/CD328 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13939-1-AP (Siglec-7/CD328 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 40x lens).

Siglec-7/CD328 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13939-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

SIGLEC7, Siglec-7, Sialic acid-binding Ig-like lectin 7, D siglec, CD328

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Tested Applications

• Positive WB detected in: human placenta tissue, human liver tissue, U-937 cells, mouse placenta tissue
• Positive IHC detected in: human tonsillitis tissue, human liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

13939-1-AP targets Siglec-7/CD328 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4220

  Product name: Recombinant human SIGLEC7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC028150

  Sequence: MLLLLLLPLLWGRERVEGQKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLL

• Full Name: sialic acid binding Ig-like lectin 7
• Calculated Molecular Weight: 467 aa, 51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC028150
• Gene Symbol: Siglec-7
• Gene ID (NCBI): 27036
• RRID: AB_10919158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y286
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Oncol Lett; Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer.; Authors - Kensuke Yamada
		FEBS Open Bio; TWIST1 induces phenotypic switching of vascular smooth muscle cells by downregulating p68 and microRNA-143/145.; Authors - Jing Zhang
human	WB,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate