SIGMAR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIGMAR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

44

Reviews (1)

Cat no : 15168-1-AP

Synonyms

Sigma 1-type opioid receptor, Sigma 1 type opioid receptor, OPRS1, hSigmaR1, Aging-associated gene 8 protein

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Validation Data Gallery

• 

  WB analysis using 15168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15168-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse kidney tissue, mouse colon tissue, rat colon tissue
• Positive IHC detected in: human liver cancer tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 37 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

15168-1-AP targets SIGMAR1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIGMAR1 fusion protein Ag7351
• Full Name: sigma non-opioid intracellular receptor 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC004899
• Gene Symbol: SIGMAR1
• Gene ID (NCBI): 10280
• RRID: AB_2301712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 28 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
human	WB	Ann Neurol; Hereditary Spastic Paraplegia-Linked REEP1 Modulates ER-Mitochondria Contacts.; Authors - Youngshin Lim
		Proc Natl Acad Sci U S A; DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1.; Authors - Yi Liu
mouse	WB	Schizophr Bull; Allosteric Modulation of the Sigma-1 Receptor Elicits Antipsychotic-like Effects.; Authors - Jiali Chen
mouse	WB	Cell Mol Life Sci; PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis.; Authors - Jessica Knoell
zebrafish	WB	Int J Mol Sci; Sigma-1 Receptor Is Critical for Mitochondrial Activity and Unfolded Protein Response in Larval Zebrafish.; Authors - Lucie Crouzier ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anastasia (Verified Customer) (08-07-2024)

SigmaR1 IF in zebrafish embryos with EGFP labeled ER

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400 (for IF)
• Cell Tissue Type: Zebrafish embryos

SIGMAR1 Antibody Immunofluorescence validation (1:400 (for IF) dilution) in Zebrafish embryos (Cat no:15168-1-AP)

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