Tested Applications
| Positive WB detected in | Sp2/0 cells, HeLa cells, SGC-7901 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17825-1-AP targets TCEA1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12199 Product name: Recombinant human SII-TFIIS,TCEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC072460 Sequence: MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC Predict reactive species |
| Full Name | transcription elongation factor A (SII), 1 |
| Calculated Molecular Weight | 301 aa, 34 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC072460 |
| Gene Symbol | TCEA1 |
| Gene ID (NCBI) | 6917 |
| RRID | AB_2201001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23193 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TCEA1 antibody 17825-1-AP | Download protocol |
| IP protocol for TCEA1 antibody 17825-1-AP | Download protocol |
| WB protocol for TCEA1 antibody 17825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TCEA1 Polyclonal antibody (Cat# 17825-1-AP) has 3 citations published in International Journal of Biological Sciences, International Journal of Molecular Sciences, and American Journal of Cancer Research. The research fields span lipid metabolism in fatty liver disease, acute promyelocytic leukemia suppression via C/EBPε and IRF8 upregulation, and hepatic carcinoma cell proliferation through the TCEA1/DDX3 axis.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.
| ||
Int J Mol Sci TCEA1 Suppresses Acute Promyelocytic Leukemia by Upregulating C/EBPε and IRF8. | ||
Am J Cancer Res Abnormal expression of YEATS4 associates with poor prognosis and promotes cell proliferation of hepatic carcinoma cell by regulation the TCEA1/DDX3 axis. |
