TCEA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TCEA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17825-1-AP

Synonyms

GTF2S, SII, SII TFIIS, TCEA, TCEA1, TF2S, TFIIS

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Validation Data Gallery

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  WB analysis of Sp2/0 using 17825-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17825-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17825-1-AP

  IP result of anti-TCEA1 (IP:17825-1-AP, 4ug; Detection:17825-1-AP 1:500) with HeLa cells lysate 520ug.

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  IHC staining of human brain using 17825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17825-1-AP (TCEA1 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human brain using 17825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17825-1-AP (TCEA1 antibody) at dilution of 1:400 (under 40x lens).

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Tested Applications

• Positive WB detected in: Sp2/0 cells, HeLa cells, SGC-7901 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17825-1-AP targets TCEA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCEA1 fusion protein Ag12199
• Full Name: transcription elongation factor A (SII), 1
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC072460
• Gene Symbol: TCEA1
• Gene ID (NCBI): 6917
• RRID: AB_2201001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

Publications

Species	Application	Title
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Am J Cancer Res; Abnormal expression of YEATS4 associates with poor prognosis and promotes cell proliferation of hepatic carcinoma cell by regulation the TCEA1/DDX3 axis.; Authors - Song You