SIK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIK1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17370-1-AP

Synonyms

MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK

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Validation Data Gallery

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  IHC staining of human testis using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 17370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17370-1-AP (SIK1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human testis tissue, human stomach tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 17370-1-AP is SIK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIK1 fusion protein Ag10954
• Full Name: salt-inducible kinase 1
• Calculated Molecular Weight: 783 aa, 85 kDa
• GenBank Accession Number: BC038504
• Gene Symbol: SIK1
• Gene ID (NCBI): 150094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832).

Publications

Species	Application	Title
mouse	WB	PLoS One; Salt-inducible kinases (SIK) inhibition reduces RANKL-induced osteoclastogenesis.; Authors - Maria Stella Lombardi ; KO Validated