Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, and mouse liver tissue |
| Positive IHC detected in | mouse brain tissue, human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51045-1-AP targets Snf1lk in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3913 Product name: Recombinant mouse Snf1lk protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 423-769 aa of NM_010831 Sequence: LDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPCIIVSSSATASPSEGTSSDSCLPFSASEGPAGLGSGLATPGLLGTSSPVRLASPFLGSQSATPVLQTQAGLGTAVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSVRTPRGGMSTFHTPAPSSGLQGCTTSNREGRSLLEEVLHQQRLLQLQHHSSTAAASSGCQQGPQLSPVPYVLAPCDSLLVSGIPLLPTPLLQAGMSPVASAAHLLDTHLHISAGPVALPTGPLPQCLTRLSPGCDPAGLPQGDCEMEDLTS Predict reactive species |
| Full Name | salt inducible kinase 1 |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 85-100 kDa |
| GenBank Accession Number | NM_010831 |
| Gene Symbol | Sik1 |
| Gene ID (NCBI) | 17691 |
| RRID | AB_2187322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q60670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Snf1lk antibody 51045-1-AP | Download protocol |
| IHC protocol for Snf1lk antibody 51045-1-AP | Download protocol |
| WB protocol for Snf1lk antibody 51045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SIK1 antibody (Cat# 51045-1-AP) has 21 citations published in journals including J Nanobiotechnology, Genes Dis, J Neuroinflammation, Phytomedicine, J Transl Med, Cell Signal, iScience, FASEB J, Inflamm Bowel Dis, J Ovarian Res, and others. Research fields span osteoarthritis, asthma, stroke, kidney disease, gluconeogenesis, gastric/ovarian/lung/pancreatic cancer, colitis, NAFLD, insulin resistance, DNA replication, inflammation, and pulmonary hypertension.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Liposome-encapsulated AICAR hydrogel regulates macrophage metabolic reprogramming via SIK1 activation to alleviate osteoarthritis | ||
Genes Dis Salt-inducible kinase 1 is a key gene in suppressing EVD68-induced asthma by modulating antiviral immunity.
| ||
J Neuroinflammation High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke. | ||
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
J Transl Med Role of SIK1 in the transition of acute kidney injury into chronic kidney disease.
| ||
Front Endocrinol (Lausanne) SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells. |
Reviews
What customers say
All three reviews are for Western Blot applications. Users reported positive results with dilutions ranging from 1:500 to 1:1000 across different cell types and tissues, including mouse lung tissue, A549 cells, and AC16 cells. Comments were generally favorable, with one user noting the antibody "worked well" and others simply rating it "Good." Average rating is approximately 4 out of 5, indicating reliable performance for WB use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
SK (Verified Customer) (01-29-2026) | Good
 |
SD (Verified Customer) (07-05-2024) | Antibody worked well
 |
P (Verified Customer) (05-16-2024) | Good
 |
