Snf1lk Polyclonal antibody

• Featured Product
• KD/KO Validated

Snf1lk Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (2)

Cat no : 51045-1-AP

Synonyms

Sik1, Salt-inducible kinase 1, salt inducible kinase 1, Myocardial SNF1-like kinase, Msk

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Validation Data Gallery

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  WB analysis of A549 using 51045-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 51045-1-AP

  WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

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  WB analysis using 51045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 51045-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: mouse brain tissue, human liver tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Product Information

51045-1-AP targets Snf1lk in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Snf1lk fusion protein Ag3913
• Full Name: salt inducible kinase 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85-100 kDa
• GenBank Accession Number: NM_010831
• Gene Symbol: Sik1
• Gene ID (NCBI): 17691
• RRID: AB_2187322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

Publications

Species	Application	Title
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
human	WB,IHC	iScience; Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus; Authors - Jie Ning ; KD Validated
human	WB, IF	J Neuroinflammation; High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.; Authors - Mengyan Hu
human	WB	J Cell Physiol; ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes; Authors - Xueyi Chen
mouse,rat	IHC,IF	J Cereb Blood Flow Metab; Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.; Authors - Cheng Jian J
human	WB	J Leukoc Biol; SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.; Authors - Maria Stella Lombardi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SD (Verified Customer) (07-05-2024)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

Snf1lk Antibody Western Blot validation (1000 dilution) in A549 (Cat no:51045-1-AP)

FH

P (Verified Customer) (05-16-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 500
• Cell Tissue Type: AC16

Snf1lk Antibody Western Blot validation (500 dilution) in AC16 (Cat no:51045-1-AP)