SIL1 Polyclonal antibody

SIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24110-1-AP

Synonyms

UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein

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Validation Data Gallery

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  WB analysis using 24110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24110-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24110-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24110-1-AP

  Immunofluorescent analysis of HepG2 cells using 24110-1-AP (SIL1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue, mouse colon tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

24110-1-AP targets SIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIL1 fusion protein Ag21367
• Full Name: SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC011568
• Gene Symbol: SIL1
• Gene ID (NCBI): 64374
• RRID: AB_2879434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.; Authors - Francesca Potenza
mouse	WB	Mol Biol Cell; Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion.; Authors - Viraj P Ichhaporia
mouse	WB	Mol Neurobiol; Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration; Authors - Yuanyuan Xu