Filter: AllWBIHCIF/ICC

WB analysis using 24110-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 24110-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse colon using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 24110-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 24110-1-AP
Immunofluorescent analysis of HepG2 cells using 24110-1-AP (SIL1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

SIL1 Polyclonal antibody

Name: SIL1 Polyclonal antibody
Brand: Proteintech
SKU: 24110-1-AP

SIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24110-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected in	human kidney tissue, mouse colon tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

24110-1-AP targets SIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21367 Product name: Recombinant human SIL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 88-461 aa of BC011568 Sequence: GSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR Predict reactive species
Full Name	SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC011568
Gene Symbol	SIL1
Gene ID (NCBI)	64374
RRID	AB_2879434
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q9H173
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SIL1 antibody 24110-1-AP	Download protocol
IHC protocol for SIL1 antibody 24110-1-AP	Download protocol
WB protocol for SIL1 antibody 24110-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Mol Sci Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss. Authors - Francesca Potenza View Article
mouse	WB	Mol Biol Cell Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion. Authors - Viraj P Ichhaporia View Article
mouse	WB	Mol Neurobiol Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration Authors - Yuanyuan Xu View Article