SIN3A Monoclonal antibody, PBS Only

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  WB analysis using 67197-1-Ig (same clone as 67197-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67197-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67197-1-Ig (same clone as 67197-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67197-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67197-1-Ig (same clone as 67197-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67197-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67197-1-Ig (same clone as 67197-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67197-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 67197-1-Ig (same clone as 67197-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SIN3A antibody (67197-1-Ig, Clone: 2B12E8 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67197-1-PBS in a different storage buffer formulation.

SIN3A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67197-1-PBS

Clone No.2B12E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DKFZp434K2235, FLJ90319, KIAA0700, SIN3A

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Product Information

67197-1-PBS targets SIN3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28865

  Product name: Recombinant human SIN3A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-156 aa of BC066364

  Sequence: MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVVFSSFSWVKKFIFSRKYWLQLVGCGGTTSNLRY

• Full Name: SIN3 homolog A, transcription regulator (yeast)
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC066364
• Gene Symbol: SIN3A
• Gene ID (NCBI): 25942
• RRID: AB_2882490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96ST3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.