SIPA1L3 Polyclonal antibody

SIPA1L3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 30544-1-AP

Synonyms

KIAA0545, SIPA1 like protein 3, SIPA1L3, SPA 1 like protein 3, SPAL3

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Validation Data Gallery

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  WB analysis using 30544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30544-1-AP

  IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of HeLa using 30544-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 30544-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30544-1-AP targets SIPA1L3 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIPA1L3 fusion protein Ag30750
• Full Name: signal-induced proliferation-associated 1 like 3
• Observed Molecular Weight: 195 kDa
• GenBank Accession Number: BC150620
• Gene Symbol: SIPA1L3
• Gene ID (NCBI): 23094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STEAP3 (Signal-induced proliferation-associated 1-like protein 3) is also named as KIAA0545 and SPAL3. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).