SIRP Alpha/CD172a Recombinant monoclonal antibody

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  WB analysis of U-937 using 83729-5-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 83729-5-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83729-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

SIRP Alpha/CD172a Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83729-5-RR

Clone No.240789C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83729-5-RR targets SIRP Alpha/CD172a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: NM_080792
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_3671328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).