SIRP Alpha Monoclonal antibody

SIRP Alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, Mouse, Rat, Pig, Rabbit







Cat no : 67337-1-Ig


BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP Alpha, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2

Tested Applications

Positive WB detected inpig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67337-1-Ig targets SIRP Alpha in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRP Alpha fusion protein Ag6361
Full Name signal-regulatory protein alpha
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC033092
Gene Symbol SIRPA
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.


Product Specific Protocols
WB protocol for SIRP Alpha antibody 67337-1-IgDownload protocol
Standard Protocols
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