SIRP Alpha/CD172a Monoclonal antibody

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8H3

Cat no : 67337-1-Ig

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Validation Data Gallery

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  WB analysis using 67337-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 67337-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67337-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67337-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 67337-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat cerebellum using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67337-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue
• Positive IHC detected in: rat cerebellum tissue, human liver tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRP Alpha/CD172a fusion protein Ag6361
• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC033092
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_2882595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.