SIRP Alpha/CD172a Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00713-1

  Sandwich ELISA standard curve of MP00713-1, Human SIRPA Recombinant Matched Antibody Pair - PBS only. 83729-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0625. 83729-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

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  Sample test of MP00713-1

  SH-SY5Y call lysate was measured.The human SIRPA concentration of detected samples was determined to be 5,156.59 pg/mL.

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  Sample test of MP00713-1

  Serum of sixteen humans was measured. The human SIRPA concentration of detected samples was determined to be 2,250 pg/mL with a range of 79.96 - 3,800.09 pg/mL.

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  Cytometric bead array standard curve of MP00713-1

  Cytometric bead array standard curve of MP00713-1, SIRP Alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83729-1-PBS. Detection antibody: 83729-4-PBS. Standard: Eg0625. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83729-4-PBS

  Biolayer interferometry (BLl) kinetic assays of 83729-4-PBS against Human SIRP Alpha/CD172a were performed. The affinity constant is 3.97 nM.

SIRP Alpha/CD172a Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83729-4-PBS

Clone No.240789G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

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Product Information

83729-4-PBS targets SIRP Alpha/CD172a as part of a matched antibody pair:

MP00713-1: 83729-1-PBS capture and 83729-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0625

  Product name: Recombinant Human SIRP alpha/CD172a protein (His Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 31-370 aa of NM_080792

  Sequence: EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_080792
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78324
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.