SIRP Beta 1 Polyclonal antibody

SIRP Beta 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11811-1-AP

Synonyms

CD172b, SIRP BETA 1, SIRPB1

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Validation Data Gallery

• 

  WB analysis using 11811-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11811-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11811-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11811-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: U-937 cells, A375 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

11811-1-AP targets SIRP Beta 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRP Beta 1 fusion protein Ag2478
• Full Name: signal-regulatory protein beta 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC025286
• Gene Symbol: SIRPB1
• Gene ID (NCBI): 10326
• RRID: AB_2188197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.

Publications

Species	Application	Title
human	IHC	PLoS One; SNPs array karyotyping reveals a novel recurrent 20p13 amplification in primary myelofibrosis.; Authors - Visani Giuseppe G
human	IHC	Neuropathology; Immunohistochemical characterization of microglia in Nasu-Hakola disease brains.; Authors - Satoh Jun-Ichi J