SIRT3 Polyclonal antibody

SIRT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29838-1-AP

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

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Validation Data Gallery

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  WB analysis using 29838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29838-1-AP (SIRT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 29838-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 29838-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29838-1-AP targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT3 fusion protein Ag31333
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC001042
• Gene Symbol: SIRT3
• Gene ID (NCBI): 23410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.