Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, PC-3 cells |
| Positive IHC detected in | human placenta tissue, mouse stomach tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28677-1-AP targets SIRT6 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29163 Product name: Recombinant human SIRT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-328 aa of BC028220 Sequence: ATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS Predict reactive species |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC028220 |
| Gene Symbol | SIRT6 |
| Gene ID (NCBI) | 51548 |
| RRID | AB_3669666 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N6T7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The SIRT6 gene is located at chromosome 19p13.3 and contains 8 exons. The encoded protein has a total length of 355 amino acids. SIRT6 protein is a member of the Sirtuins, which is a class of NAD+-dependent protein deacetylases involved in stress resistance and metabolic homeostasis. SIRT6 also plays roles in various tumors as both an oncogene and tumor suppressor gene. A previous study in osteosarcoma reported that SIRT6 regulates the migration and invasion of tumors through the ERK1/2/MMP9 pathway. (PMID: 30675128)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SIRT6 antibody 28677-1-AP | Download protocol |
| IHC protocol for SIRT6 antibody 28677-1-AP | Download protocol |
| WB protocol for SIRT6 antibody 28677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SIRT6 antibody (Cat# 28677-1-AP) has 3 total citations published in Redox Biology, Advanced Healthcare Materials, and Molecular and Cellular Biochemistry. The associated research fields include diabetic angiopathy and glycolysis reprogramming, cartilage senescence and cellular uptake remodeling, and hepatocellular carcinoma chemoresistance via SIRT6-mediated ERK1 deacetylation inhibition. Applications cited include IF, CoIP, and IHC across human and mouse species.
| Species | Application | Title |
|---|---|---|
Redox Biol Translocation of SIRT6 promotes glycolysis reprogramming to exacerbate diabetic angiopathy. | ||
Adv Healthc Mater Remodeling Cellular Uptake via Inducing Clustering of Urokinase Receptors in Hydrogel Microspheres for Alleviating Cartilage Senescence. | ||
Mol Cell Biochem Squalene attenuates doxorubicin resistance in hepatocellular carcinoma by targeting SIRT6 to inhibit ERK1 deacetylation. |
