SIRT6 Recombinant monoclonal antibody, PBS Only

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• SIRT6 Recombinant monoclonal antibody, PBS Only (87677-3-PBS)

SIRT6 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 87677-3-PBS

Clone No.253025G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6, NAD-dependent protein deacetylase sirtuin-6

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  WB analysis using 87677-3-RR (same clone as 87677-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87677-3-RR (SIRT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 87677-3-RR (same clone as 87677-3-PBS)

  IP result of anti-SIRT6 (IP:87677-3-RR, 4ug; Detection:87677-3-RR 1:2000) with HeLa cells lysate 920 ug. This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 87677-3-RR (same clone as 87677-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87677-3-RR (SIRT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 87677-3-RR (same clone as 87677-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87677-3-RR (SIRT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87677-3-PBS targets SIRT6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4469

  Product name: Recombinant human SIRT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC028220

  Sequence: MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSNVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS

• Full Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC028220
• Gene Symbol: SIRT6
• Gene ID (NCBI): 51548
• RRID: AB_3745691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N6T7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).