SIRT7 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

  IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84805-4-RR (same clone as 84805-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SIRT7 antibody (84805-4-RR, Clone: 242167C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84805-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

SIRT7 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 84805-4-PBS

Clone No.242167C9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

242167C9, EC:2.3.1.-, EC:2.3.1.286, NAD-dependent protein deacylase sirtuin-7, SIR2 like protein 7

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Product Information

84805-4-PBS targets SIRT7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag31794

  Product name: Recombinant human SIRT7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of NM_016538

  Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

• Full Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Calculated Molecular Weight: 45KD
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_016538
• Gene Symbol: SIRT7
• Gene ID (NCBI): 51547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NRC8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.