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SIX2 Polyclonal antibody

SIX2 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG


human and More (6)





Cat no : 11562-1-AP


Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

Tested Applications

Positive IP detected inHEK-293 cells
Positive IF detected inmouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, rabbit, pig, Alligator, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIX2 fusion protein Ag2124
Full Name SIX homeobox 2
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.


Product Specific Protocols
WB protocol for SIX2 antibody 11562-1-APDownload protocol
IP protocol for SIX2 antibody 11562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: E18.5 kidney
SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)