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  • KD/KO Validated

SIX2 Polyclonal antibody

SIX2 Polyclonal Antibody for IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11562-1-AP

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2



Tested Applications

Positive IP detected inHEK-293 cells
Positive IF detected inmouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, Alligator, canine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIX2 fusion protein Ag2124
Full Name SIX homeobox 2
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC024033
Gene symbol SIX2
Gene ID (NCBI) 10736
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Protocols

Product Specific Protocols
WB protocol for SIX2 antibody 11562-1-APDownload protocol
IP protocol for SIX2 antibody 11562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.

Authors - Minoru Takasato
humanIF

Nat Biotechnol

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Authors - Frans Schutgens
humanIF

Nat Biotechnol

Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.

Authors - Ravian L van Ineveld
mouseIF

Cell

In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.

Authors - Hsin-Kai Liao
humanIF

Cell

Tridimensional Visualization and Analysis of Early Human Development.

Authors - Morgane Belle
mouseIF

Cell Stem Cell

Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells.

Authors - Atsuhiro Taguchi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: E18.5 kidney
SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)