Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, alligator |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2124 Product name: Recombinant human SIX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-291 aa of BC024033 Sequence: MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS Predict reactive species |
| Full Name | SIX homeobox 2 |
| Calculated Molecular Weight | 291 aa, 32 kDa |
| Observed Molecular Weight | 32-37 kDa |
| GenBank Accession Number | BC024033 |
| Gene Symbol | SIX2 |
| Gene ID (NCBI) | 10736 |
| RRID | AB_2189084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SIX2 antibody 11562-1-AP | Download protocol |
| WB protocol for SIX2 antibody 11562-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SIX2 (Cat# 11562-1-AP) has 307 citations published in journals including Nature, Cell, Cell Stem Cell, Nature Communications, Nature Protocols, Development, J Am Soc Nephrol, Kidney International, and Science Advances. Research fields span kidney development and nephrogenesis, kidney organoid generation, stem cell biology, Wilms tumor, nephron progenitor cell regulation, regenerative medicine, and signaling pathways (Wnt, Notch, FGF, Hedgehog).
| Species | Application | Title |
|---|---|---|
Nature Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. | ||
Nature A NIK-SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-κB. | ||
Cell In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. | ||
Nat Biotechnol Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D. | ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. |
Reviews
What customers say
Customers rate this antibody favorably across applications. In Western Blot on HEK-293 cells, reviewers consistently report strong, reliable performance, describing it as "working very good." For Immunofluorescence on E18.5 kidney tissue, a user achieved excellent results at a 1/200 dilution, noting successful antigen retrieval (boiling in 10 mM citrate buffer, pH 6, for 12 minutes). Overall, the antibody is well-regarded for both WB and IF, with clear, specific signals and ease of use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Prakash (Verified Customer) (11-16-2025) | Working very good in western blot
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Prakash (Verified Customer) (10-28-2025) | works good in western blot.
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Prakash (Verified Customer) (10-28-2025) | works good in western blot
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Nicolas (Verified Customer) (09-17-2020) | I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)
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