|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, pig, Alligator, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIX2 fusion protein Ag2124|
|Full Name||SIX homeobox 2|
|Calculated molecular weight||291 aa, 32 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||BC024033|
|Gene ID (NCBI)||10736|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nicolas (Verified Customer) (09-17-2020)
I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)