SIX6OS1 Polyclonal antibody

• 

  WB analysis using 30610-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30610-1-AP (SIX6OS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SIX6OS1 Polyclonal Antibody for WB, ELISA

Cat No. 30610-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C14orf39, Protein SIX6OS1, Six6 opposite strand transcript 1

---

Tested Applications

• Positive WB detected in: COLO 320 cells, Jurkat cells, SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30610-1-AP targets SIX6OS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33548

  Product name: Recombinant human C14orf39 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 200-420 aa of BC125070

  Sequence: SNLTKSSSELKKEVDEMEIEINYLNQQISRHNETKALSETLEEKNKNTENRKELKERIFGKDEHVLTLNKTQSSQLFLPYESQKLVRPIKMHSSEPRVADIKEESSAKQSKLANIDFRQKENDTQIFNDSAVDNHSKCSHITTITSSQKFMQVRLLTPQKQSNSNQWSEKGDKDAEYGDKGTVRQVRESKCTSQAIYTEHFGKSIENDSDEVEERAENFPR

• Full Name: chromosome 14 open reading frame 39
• Calculated Molecular Weight: 587 aa, 68 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC125070
• Gene Symbol: C14orf39
• Gene ID (NCBI): 317761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N1H7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX6OS1 (SIX6 Opposite Strand 1) is a nuclear protein that plays a core role in the process of meiosis. It is a key scaffold protein that constitutes the central element of the synaptonemal complex, directly interacting with proteins such as SYCE1 to ensure the proper pairing, synapsis, and genetic recombination of homologous chromosomes. Its function is crucial for the formation of stable chiasmata, which are the basis for genetic diversity and the accurate segregation of chromosomes. Mutations in the SIX6OS1 gene can disrupt this process, leading to errors in chromosome separation and are one of the important genetic factors causing primary ovarian insufficiency in humans and non-obstructive azoospermia in males, directly associated with reproductive disorders.