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SKIL Polyclonal antibody, PBS Only (19218-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 19218-1-AP (same clone as 19218-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 19218-1-AP (same clone as 19218-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SKIL Polyclonal antibody, PBS Only

Name: SKIL Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 19218-1-PBS

SKIL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19218-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

19218-1-PBS targets SKIL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5282 Product name: Recombinant human SKIL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-684 aa of BC059386 Sequence: YLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKSESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVKMLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYAGQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE Predict reactive species
Full Name	SKI-like oncogene
Calculated Molecular Weight	77 kDa
Observed Molecular Weight	77 kDa
GenBank Accession Number	BC059386
Gene Symbol	SKIL
Gene ID (NCBI)	6498
RRID	AB_10859824
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12757
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.