SKOR2 Polyclonal antibody

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  WB analysis using 55370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55370-1-AP (SKOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 55370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 55370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 55370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SKOR2 antibody (55370-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SKOR2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 55370-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

FUSSEL18

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55370-1-AP targets SKOR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: functional smad suppressing element 18
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 30 kDa, 60 kDa
• GenBank Accession Number: XM_001715219
• Gene Symbol: SKOR2
• Gene ID (NCBI): 652991
• RRID: AB_3086446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2VWA4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKOR2 (SKI family transcriptional corepressor 2), also named FUSSEL18, is a transcriptional repressor that is highly expressed in the central nervous system (PMID: 16200078). SKOR2 was detected at 30 and 60 kD in the cerebellum. It is identified as a novel transcriptional regulator in Purkinje cells that acts upstream of Shh during cerebellum development (PMID: 21937600). Skor2 was detected in Purkinje cells in the human fetal cerebellum (PMID: 34140698).