SLAM/CD150 Polyclonal antibody

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  WB analysis of Jurkat using 10949-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10949-2-AP (SLAM/CD150 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

SLAM/CD150 Polyclonal Antibody for WB, ELISA

Cat No. 10949-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10949-2-AP targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1386

  Product name: Recombinant human SLAMF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-144 aa of BC012602

  Sequence: MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKSLHFGYLYFVLGADHDNLQT

• Full Name: signaling lymphocytic activation molecule family member 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC012602
• Gene Symbol: CD150
• Gene ID (NCBI): 6504
• RRID: AB_2187822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13291
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.