SLC12A1/NKCC2 Recombinant monoclonal antibody

SLC12A1/NKCC2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87210-1-RR

Clone No. 252363D10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

NKCC2, SLC12A1, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 87210-1-RR
Untreated and 37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 87210-1-RR (SLC12A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 87210-1-RR
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 87210-1-RR (SLC12A1/NKCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 87210-1-RR
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 87210-1-RR (SLC12A1/NKCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SLC12A1/NKCC2 in ?

Positive WB detected in	37°C incubated mouse kidney tissue
Positive IHC detected in	human kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

87210-1-RR targets SLC12A1/NKCC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag5325 Product name: Recombinant human SLC12A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-176 aa of BC040138 Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVK Predict reactive species
Full Name	solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Calculated Molecular Weight	121 kDa
Observed Molecular Weight	140-180 kDa
GenBank Accession Number	BC040138
Gene Symbol	SLC12A1/NKCC2
Gene ID (NCBI)	6557
RRID	AB_3745408
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13621
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.