SLC17A5 Polyclonal antibody

SLC17A5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13320-1-AP

Synonyms

AST, ISSD, Membrane glycoprotein HP59, NSD, SD, SIALIN, SIASD, SLC17A5, SLD

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Validation Data Gallery

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  WB analysis of human kidney using 13320-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13320-1-AP (SLC17A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13320-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13320-1-AP (SLC17A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human kidney tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13320-1-AP targets SLC17A5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC17A5 fusion protein Ag3855
• Full Name: solute carrier family 17 (anion/sugar transporter), member 5
• Calculated Molecular Weight: 495 aa, 55 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC020961
• Gene Symbol: SLC17A5
• Gene ID (NCBI): 26503
• RRID: AB_2187524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC17A5 encodes a transmembrane protein, sialin, which transports free sialic acid across the lysosomal membrane. Mutations in sialin result in neurodegenerative sialic acid storage disorders, Salla disease (SD) and infantile sialic acid storage disease (ISSD). In the central nervous system, sialin is more abundant in neurons than in glia. It has been reported to be localized into lysosome in neuron but in plasma membrane in nonneuronal cells (PMID: 15006695).