SLC18A1 Polyclonal antibody

SLC18A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20340-1-AP

Synonyms

CGAT, SLC18A1, VAT1, Vesicular amine transporter 1, VMAT1

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Validation Data Gallery

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  WB analysis of HEK-293 using 20340-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20340-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20340-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 20340-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20340-1-AP (SLC18A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20340-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20340-1-AP (SLC18A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 20340-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20340-1-AP (SLC18A1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, C6 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20340-1-AP is SLC18A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC18A1 fusion protein Ag14131
• Full Name: solute carrier family 18 (vesicular monoamine), member 1
• Calculated Molecular Weight: 525 aa, 56 kDa
• Observed Molecular Weight: 50 kDa, 56 kDa
• GenBank Accession Number: BC009387
• Gene Symbol: SLC18A1
• Gene ID (NCBI): 6570
• RRID: AB_10666128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.