SLC19A1 Polyclonal antibody

SLC19A1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25958-1-AP

Synonyms

CHMD, FLOT1, Folate transporter 1, FOLT, IFC 1, IFC1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, REFC, RFC, RFC1, SLC19A1



Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells
Positive IHC detected inhuman placenta tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25958-1-AP is SLC19A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC19A1 fusion protein Ag22921
Full Name solute carrier family 19 (folate transporter), member 1
Calculated molecular weight 591 aa, 65 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC003068
Gene symbol SLC19A1
Gene ID (NCBI) 6573
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC19A1, also named as RFC (reduced folate carrier), is an anionic exchanger that involved in the regulation of intracellular concentrations of folate in exchange for anions. The predicted MW of  SLC19A1 is 65 kDa and 25958-1-AP antibody recognizes the 46 kDa protein which is similar to papers. The 38-40 kDa minor forms and 58 kDa form have also been reported that could be alternate splicing, post-transcriptional modification or the role of accessory proteins. (PMID: 17404734, 9111015)

Protocols

Product Specific Protocols
WB protocol for SLC19A1 antibody 25958-1-APDownload protocol
IHC protocol for SLC19A1 antibody 25958-1-APDownload protocol
Standard Protocols
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