SLC19A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC19A1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 25958-1-AP

Synonyms

CHMD, FLOT1, Folate transporter 1, FOLT, IFC 1, IFC1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, REFC, RFC, RFC1, SLC19A1

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Validation Data Gallery

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  WB analysis using 25958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25958-1-AP (SLC19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells
• Positive IHC detected in: human placenta tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

25958-1-AP targets SLC19A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC19A1 fusion protein Ag22921
• Full Name: solute carrier family 19 (folate transporter), member 1
• Calculated Molecular Weight: 591 aa, 65 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC003068
• Gene Symbol: SLC19A1
• Gene ID (NCBI): 6573
• RRID: AB_2880310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC19A1, also named as RFC (reduced folate carrier), is an anionic exchanger that involved in the regulation of intracellular concentrations of folate in exchange for anions. The predicted MW of  SLC19A1 is 65 kDa and 25958-1-AP antibody recognizes the 46 kDa protein which is similar to papers. The 38-40 kDa minor forms and 58 kDa form have also been reported that could be alternate splicing, post-transcriptional modification or the role of accessory proteins. (PMID: 17404734, 9111015)

Publications

Species	Application	Title
mouse	WB	J Clin Invest; An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP; Authors - Xiaobo Li ; KD Validated
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

It does not show a clear band around 46kDa for SLC19A1. It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: HepG2, mouse liver and brain

SLC19A1 Antibody Western Blot validation (1:400 dilution) in HepG2, mouse liver and brain (Cat no:25958-1-AP)