Tested Applications
| Positive WB detected in | mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse kidney tissue, human placenta tissue, rat heart tissue, 3T3-L1 cells, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13407-1-AP targets SLC19A3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4053 Product name: Recombinant human SLC19A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-315 aa of BC032014 Sequence: LFLPMPKKSMFFHAKPSREIKKSSSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFYWSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIY Predict reactive species |
| Full Name | solute carrier family 19, member 3 |
| Calculated Molecular Weight | 496 aa, 56 kDa |
| Observed Molecular Weight | 63-70 kDa |
| GenBank Accession Number | BC032014 |
| Gene Symbol | SLC19A3 |
| Gene ID (NCBI) | 80704 |
| RRID | AB_2187997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZV2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SLC19A3 antibody 13407-1-AP | Download protocol |
| WB protocol for SLC19A3 antibody 13407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SLC19A3 (Cat# 13407-1-AP) has 13 citations across journals including Nature Communications, Science Translational Medicine, Redox Biology, Journal of Biological Chemistry, American Journal of Physiology (Cell & Gastrointestinal), Frontiers in Pharmacology, Frontiers in Cell and Developmental Biology, Journal of Nutritional Biochemistry, Neurotoxicology Research, PLoS One, and Obesity Science & Practice. Research fields span thiamine/vitamin B1 transport and metabolism, neurodegeneration (Huntington's disease, basal ganglia encephalopathy), alcohol-induced brain damage, intestinal physiology, oxidative stress, breast cancer, placental biology, and obesity.
| Species | Application | Title |
|---|---|---|
Nat Commun Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia | ||
Redox Biol Clioquinol inactivates thiamine pyrophosphate by increasing cellular oxidative stress. | ||
Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. | ||
Am J Physiol Cell Physiol Bacterial lipopolysaccharide inhibits free thiamin uptake along the intestinal tract via interference with membrane expression of thiamin transporters-1 & -2 | ||
Am J Physiol Cell Physiol Tumor necrosis factor α impedes colonic thiamin pyrophosphate and free thiamin uptake: involvement of JNK/ERK1/2-mediated pathways | ||
Front Pharmacol Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice. |
Reviews
What customers say
All three reviewers rate this antibody favorably (average ~4.7/5). One long-time user (5+ years) reports consistent performance in Western blot on colonic cell lines and tissue at 1:1000 dilution and plans to continue using it. Another highlights highly reliable results across Western blot, immunofluorescence, and protein purification. A third confirms frequent use in their lab for Western blot on human intestinal cell lines. Overall, users praise its reliability and consistency across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Selvaraj (Verified Customer) (03-19-2026) | We have been using this for more than 5 years, it consistently works well. We would like to continue this for the next project too.
|
Valliammai (Verified Customer) (02-15-2023) | Results are highly reliable. Thanks for the service.
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Selvaraj (Verified Customer) (12-20-2022) | We are frequently using this antibody in our lab
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