EAAT3 Polyclonal antibody

EAAT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12686-1-AP

Synonyms

EAAC1, EAAT3, SLC1A1

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Validation Data Gallery

• 

  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12686-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, human brain tissue, mouse testis tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12686-1-AP targets EAAT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, pig, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EAAT3 fusion protein Ag3379
• Full Name: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• Calculated Molecular Weight: 524 aa, 57 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC033040
• Gene Symbol: EAAT3
• Gene ID (NCBI): 6505
• RRID: AB_2188001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Cancer Res; Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer.; Authors - Wenzheng Guo
mouse	WB	Life Sci; Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction; Authors - Yu Ma
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang
mouse	WB	J Alzheimers Dis; S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes.; Authors - Xinkun Wan
goat	IHC	Anim Sci J; The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high-grain diet; Authors - Changxin Tian
mouse	WB	Nutrients; L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway; Authors - Kehong Liu