SLC20A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC20A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 12423-1-AP

Synonyms

PIT1, Phosphate transporter 1, Leukemia virus receptor 1 homolog, GLVR-1, GLVR1

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Validation Data Gallery

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  WB analysis of HEK-293 using 12423-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12423-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12423-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12423-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12423-1-AP

  IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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  IHC staining of human liver using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human kidney tissue, Jurkat cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver tissue, human breast cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

12423-1-AP targets SLC20A1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC20A1 fusion protein Ag3084
• Full Name: solute carrier family 20 (phosphate transporter), member 1
• Calculated Molecular Weight: 679 aa, 74 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC019944
• Gene Symbol: SLC20A1
• Gene ID (NCBI): 6574
• RRID: AB_2286212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

Publications

Species	Application	Title
mouse	FC	Nat Cancer; GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity; Authors - Dionysios C Watson
human,mouse	WB,CoIP	Theranostics; SGK3 promotes vascular calcification via Pit-1 in chronic kidney disease; Authors - Qing-Qing Dong
human	WB	Adv Healthc Mater; Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds; Authors - Wei Chen ; KD Validated
human	WB	FASEB J; PMID: 27565711; Authors - Tuyet Thi Nguyen
zebrafish	IF	Front Cell Dev Biol; SLC20A1 Is Involved in Urinary Tract and Urorectal Development.; Authors - Johanna Magdalena Rieke
human	WB	J Biol Chem; Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake.; Authors - Nina Bon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size as a smear. The protein undergoes heavy glycosylation, so that is expected. No additional background bands were observed around the size range.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

SLC20A1 Antibody Western Blot, validation (1:500 dilution) in HEK293T (Cat no:12423-1-AP)