URAT1 Polyclonal antibody

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  WB analysis using 14937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

URAT1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14937-1-AP

Publications(94)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Synonyms

SLC22A12, RST, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein

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Tested Applications

• Positive WB detected in: mouse heart tissue, pig kidney tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 86 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Product Information

14937-1-AP targets URAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6770

  Product name: Recombinant human URAT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-351 aa of BC053348

  Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC

• Full Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65-70 kDa, 40 kDa
• GenBank Accession Number: BC053348
• Gene Symbol: URAT1
• Gene ID (NCBI): 116085
• RRID: AB_2191270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96S37
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
human,mouse	WB	Phytomedicine; The inhibitory effect of Astragalus flavone extract on hyperuricemia and its underlying molecular mechanism by targeting JNK/AP-1/NLRP3/IL-1β signaling pathway; Authors - Hongjuan Xia
rat	WB	Phytomedicine; Mechanistic insights into the potentiation and toxicity mitigation of myocardial infarction treatment with salvianolate and ticagrelor; Authors - Yan Wan
mouse	WB	Mol Metab; URAT1-selective inhibition ameliorates insulin resistance by attenuating diet-induced hepatic steatosis and brown adipose tissue whitening in mice.; Authors - Yoshiro Tanaka
mouse	IHC	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
mouse	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang