URAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

URAT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

66

Cat no : 14937-1-AP

Synonyms

OAT4L, OATL4, Renal specific transporter, RST, SLC22A12, URAT1, Urate anion exchanger 1

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Validation Data Gallery

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  WB analysis using 14937-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, pig kidney tissue, rat kidney

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 61 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

14937-1-AP targets URAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: URAT1 fusion protein Ag6770
• Full Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65-70 kDa, 40 kDa
• GenBank Accession Number: BC053348
• Gene Symbol: SLC22A12
• Gene ID (NCBI): 116085
• RRID: AB_2191270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
mouse	WB	Mol Metab; URAT1-selective inhibition ameliorates insulin resistance by attenuating diet-induced hepatic steatosis and brown adipose tissue whitening in mice.; Authors - Yoshiro Tanaka
mouse	IHC	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
mouse	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
mouse	WB	Nutrients; Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet; Authors - Nan Ma