URAT1 Polyclonal antibody

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• URAT1 Polyclonal antibody (14937-1-AP)

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URAT1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 14937-1-AP Copied!

Publications (127)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

SLC22A12, OAT4L, OATL4, Organic anion transporter 4-like protein, Renal specific transporter

• 

  WB analysis using 14937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse kidney using 14937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using URAT1 antibody (14937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse kidney using 14937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using URAT1 antibody (14937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, pig kidney tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14937-1-AP targets URAT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6770

  Product name: Recombinant human URAT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-351 aa of BC053348

  Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC

• Full Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65-70 kDa, 40 kDa
• GenBank Accession Number: BC053348
• Gene Symbol: URAT1
• Gene ID (NCBI): 116085
• RRID: AB_2191270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96S37
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publications

What published studies show

Anti-URAT1 antibody (Cat# 14937-1-AP) has accumulated 127 citations published in journals such as Phytomedicine, J Ethnopharmacol, Food Funct, J Agric Food Chem, Front Pharmacol, Nutrients, Biomed Pharmacother, Int J Biol Macromol, Eur J Pharmacol, and J Adv Res. The associated research fields predominantly include hyperuricemia, gout, uric acid metabolism and transport, renal injury, gut microbiota modulation, inflammation, and natural product pharmacology.

Automatically generated from the 127 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Adv Res; Punicalagin attenuates hyperuricemia via restoring hyperuricemia-induced renal and intestinal dysfunctions; Authors - Qing-Qing Han
mouse	WB	J Neuroinflammation; Urate inhibits microglia activation to protect neurons in an LPS-induced model of Parkinson's disease.; Authors - Li-Hui Bao
rat	WB	Phytomedicine; Mechanistic insights into the potentiation and toxicity mitigation of myocardial infarction treatment with salvianolate and ticagrelor; Authors - Yan Wan
mouse,human	WB	Phytomedicine; The inhibitory effect of Astragalus flavone extract on hyperuricemia and its underlying molecular mechanism by targeting JNK/AP-1/NLRP3/IL-1β signaling pathway; Authors - Hongjuan Xia
mouse	WB	Phytomedicine; Oxyberberrubine, a novel liver microsomes-mediated secondary metabolite of berberine, alleviates hyperuricemic nephropathy in mice; Authors - Linjiang Zhong
mouse	WB	Phytomedicine; Data mining-guided alleviation of hyperuricemia by Paeonia veitchii Lynch through inhibition of xanthine oxidase and regulation of renal urate transporters; Authors - Jiana Du