SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24901-1-AP

Synonyms

BOCT2, C6orf85, SLC22A23

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Validation Data Gallery

• 

  WB analysis of L02 using 24901-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24901-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 24901-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24901-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 24901-1-AP

  IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 24901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24901-1-AP is SLC22A23 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A23 fusion protein Ag19036
• Full Name: solute carrier family 22, member 23
• Calculated Molecular Weight: 686 aa, 74 kDa
• Observed Molecular Weight: 66-74 kDa
• GenBank Accession Number: BC128580
• Gene Symbol: SLC22A23
• Gene ID (NCBI): 63027
• RRID: AB_2879787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.