SLC25A15 Polyclonal antibody, PBS Only

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• SLC25A15 Polyclonal antibody, PBS Only (15317-1-PBS)

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  WB analysis of HeLa using 15317-1-AP (same clone as 15317-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 15317-1-AP (same clone as 15317-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse stomach using 15317-1-AP (same clone as 15317-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15317-1-AP (same clone as 15317-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

SLC25A15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15317-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

15317-1-PBS targets SLC25A15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7563

  Product name: Recombinant human SLC25A15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC002702

  Sequence: MKSNPAIQAAIDLTAGAAGGTACVLTGQPFDTMKVKMQTFPDLYRGLTDCCLKTYSQVGFRGFYKGTSPALIANIAENSVLFMCYGFCQQVVRKVAGLDKQAKLSDLQNAAAGSFASAFAALVLCPTELVKCRLQTMYEMETSGKIAKSQNTVWSVIKSILRKDGPLGFYHGLSSTLLREVPGYFFFFGGYELSRSFFASGRSKDELGPVPLMLSGGVGGICLWLAVYPVDCIKSRIQVLSMSGKQAGFIRTFLNVVKNEGITALYSGLKPTMIRAFPANGALFLAYEYSRKLMMNQLEAY

• Full Name: solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC002702
• Gene Symbol: SLC25A15
• Gene ID (NCBI): 10166
• RRID: AB_2189758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y619
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.