SLC25A3 Polyclonal antibody

SLC25A3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15855-1-AP

Synonyms

OK/SW cl.48, PHC, phosphate carrier, Phosphate transport protein, PiC, PTP, SLC25A3

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Validation Data Gallery

• 

  WB analysis of mouse pancreas using 15855-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15855-1-AP (SLC25A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15855-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15855-1-AP (SLC25A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15855-1-AP

  IP result of anti-SLC25A3 (IP:15855-1-AP, 4ug; Detection:15855-1-AP 1:500) with mouse heart tissue lysate 4400ug.

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, MCF-7 cells
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• RIP: See 1 publications below

Product Information

15855-1-AP targets SLC25A3 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A3 fusion protein Ag8698
• Full Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC000998
• Gene Symbol: SLC25A3
• Gene ID (NCBI): 5250
• RRID: AB_2190058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
pig	WB	J Biol Chem; A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction.; Authors - Xiaomin Zhao
mouse	WB	Am J Physiol Cell Physiol; miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart.; Authors - Baseler Walter A WA
pig	WB,RIP	Vet Microbiol; circEZH2 inhibits opening of mitochondrial permeability transition pore via interacting with PiC and up-regulating RSAD2.; Authors - Jianxiong Guo ; KD Validated