SLC27A3 Polyclonal antibody

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• SLC27A3 Polyclonal antibody (12943-1-AP)

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• KD/KO Validated

SLC27A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12943-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3

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  WB analysis using 12943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 12943-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 12943-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 12943-1-AP

  IP result of anti-SLC27A3 (IP:12943-1-AP, 4ug; Detection:12943-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, MCF-7 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human skin cancer tissue, human breast cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12943-1-AP targets SLC27A3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4015

  Product name: Recombinant human SLC27A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 500-700 aa of BC029792

  Sequence: GEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI

• Full Name: solute carrier family 27 (fatty acid transporter), member 3
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC029792
• Gene Symbol: SLC27A3
• Gene ID (NCBI): 11000
• RRID: AB_2286200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5K4L6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).

Publications

What published studies show

Anti-SLC27A3 antibody (Cat# 12943-1-AP) has 10 total citations spanning journals including Nature Medicine, J Neuroinflammation, Frontiers in Immunology, European Journal of Pharmacology, International Journal of Molecular Sciences, Cancers, Molecular Reproduction and Development, Biochemical and Biophysical Research Communications, bioRxiv, and Advanced Science. Research fields cover lipid metabolism, insulin resistance, neuroinflammation, COPD, hepatic cholestasis, adipose browning, breast cancer, reproductive biology, pancreatic cancer, KRAS-targeted therapy, and glioblastoma.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Med; PMID: 26950361; Authors - Cholsoon Jang ; KD Validated
		J Neuroinflammation; SLC27A3-dependent lipid metabolic reprogramming by trilobatin suppresses microglial mtDNA/TLR9-driven inflammatory activation in traumatic brain injury.; Authors - Hui-Wen Zhang
human,mouse	WB,IHC	Front Immunol; Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease.; Authors - Yuepeng Zhang
mouse	WB	Eur J Pharmacol; A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate; Authors - Yuanli Chen
mouse	WB	Int J Mol Sci; Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development; Authors - Mingfa Ling
mouse	WB	Cancers (Basel); In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer.; Authors - Megan C Madonna