SLC27A3 Recombinant monoclonal antibody

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  WB analysis using 86047-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86047-1-RR (SLC27A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 86047-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86047-1-RR (SLC27A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86047-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86047-1-RR (SLC27A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86047-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86047-1-RR against Human SLC27A3 were performed. The affinity constant is 0.342 nM.

SLC27A3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86047-1-RR

Clone No.250628D5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

ACSVL3, Arachidonate--CoA ligase, EC:6.2.1.-, EC:6.2.1.15, EC:6.2.1.3

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Tested Applications

• Positive WB detected in: MCF-7 cells, rat testis tissue, THP-1 cells, HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86047-1-RR targets SLC27A3 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4015

  Product name: Recombinant human SLC27A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 500-700 aa of BC029792

  Sequence: GEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI

• Full Name: solute carrier family 27 (fatty acid transporter), member 3
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC029792
• Gene Symbol: SLC27A3
• Gene ID (NCBI): 11000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5K4L6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).