SLC30A1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82895-2-RR (same clone as 82895-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82895-2-RR (SLC30A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82895-2-RR (same clone as 82895-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SLC30A1 Recombinant antibody (82895-2-RR, Clone:230152E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82895-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82895-2-RR against Human SLC30A1 were performed. The affinity constant is 1.49 nM.

SLC30A1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82895-2-PBS

Clone No.230152E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

230152E10, Zinc transporter 1, ZNT1, ZnT-1

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Product Information

82895-2-PBS targets SLC30A1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18567

  Product name: Recombinant human SLC30A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-507 aa of BC121015

  Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL

• Full Name: solute carrier family 30 (zinc transporter), member 1
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 63~75 kDa
• GenBank Accession Number: BC121015
• Gene Symbol: SLC30A1
• Gene ID (NCBI): 7779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6M5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319).