ZnT3 Polyclonal antibody

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• ZnT3 Polyclonal antibody (17363-1-AP)

ZnT3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17363-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

SLC30A3, Zinc transporter 3, ZnT 3, ZNT3

• 

  WB analysis using 17363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17363-1-AP (ZnT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17363-1-AP (ZnT3 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17363-1-AP targets ZnT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10735

  Product name: Recombinant human SLC30A3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 288-388 aa of BC028358

  Sequence: VLRILMEGTPRNVGFEPVRDTLLSVPGVRATHELHLWALTLTYHVASAHLAIDSTADPEAVLAEASSRLYSRFGFSSCTLQVEQYQPEMAQCLRCQEPPQA

• Full Name: solute carrier family 30 (zinc transporter), member 3
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 42 kDa, 48 kDa
• GenBank Accession Number: BC028358
• Gene Symbol: ZnT3
• Gene ID (NCBI): 7781
• RRID: AB_2239335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99726
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.

Publications

What published studies show

The ZnT3 Polyclonal antibody (Cat# 17363-1-AP) has 19 citations published in journals including Advanced Science, Molecular Medicine, Cell Biosciences, Acta Neuropathologica Communications, International Journal of Molecular Sciences, Scientific Reports, Journal of Cerebral Blood Flow & Metabolism, Brain Research Bulletin, Frontiers in Molecular Neuroscience, Journal of Biological Chemistry, Journal of Neuroscience Research, Genes, Life Science Alliance, Prostate, Biochemical & Biophysical Research Communications, Journal of Comparative Neurology, Journal of Reproduction and Development, and Biological Open. Research fields span zinc homeostasis, neurodegeneration (ALS, Huntington's, Alzheimer's), epilepsy, cognitive dysfunction, bone regeneration, oxidative stress, and reproductive biology.

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Ion-Driven Regulation of Ca(2) (+) Flow by Ti/Zn Composite Activates ERK/MAPK Signaling and Maintains Mitochondrial Homeostasis to Promote Macrophage-Mediated Bone Regeneration.; Authors - Wanyi Huang
mouse	WB,IF	Mol Med; Estrogen improves sevoflurane-induced cognitive dysfunction by regulating synaptic zinc homeostasis.; Authors - Feixiang Li
mouse	IHC	Cell Biosci; Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington's disease transgenic mouse.; Authors - Li Niu
mouse	WB	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman
human	WB	Int J Mol Sci; MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis.; Authors - Hisaka Kurita
human	IHC	Sci Rep; Putative long range mossy fiber sprouting and regional cytochrome c oxidase alteration in the hippocampus of patients with mesial temporal lobe epilepsy.; Authors - Tian Tu