ZnT3 Polyclonal antibody

ZnT3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17363-1-AP

Synonyms

SLC30A3, Zinc transporter 3, ZnT 3, ZNT3



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17363-1-AP targets ZnT3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZnT3 fusion protein Ag10735
Full Name solute carrier family 30 (zinc transporter), member 3
Calculated molecular weight 388 aa, 42 kDa
Observed molecular weight 42 kDa, 48 kDa
GenBank accession numberBC028358
Gene symbol SLC30A3
Gene ID (NCBI) 7781
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.

Protocols

Product Specific Protocols
WB protocol for ZnT3 antibody 17363-1-APDownload protocol
IHC protocol for ZnT3 antibody 17363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Neuropathol Commun

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Authors - Alexandra Grubman
mouseWB

Biol Open

Altered biometal homeostasis is associated with CLN6 mRNA loss in mouse neuronal ceroid lipofuscinosis.

Authors - Kanninen Katja M KM
humanWB,IF

Biochem Biophys Res Commun

Protective roles of SLC30A3 against endoplasmic reticulum stress via ERK1/2 activation.

Authors - Hisaka Kurita
human, mouseWB, IHC, IF

J Neurosci Res

Zinc transporters ZnT3 and ZnT6 are downregulated in the spinal cords of patients with sporadic amyotrophic lateral sclerosis.

Authors - Masayuki Kaneko
humanWB

Int J Mol Sci

MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis.

Authors - Hisaka Kurita
mouseIHC

Cell Biosci

Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington's disease transgenic mouse.

Authors - Li Niu