ZNT4 Polyclonal antibody

ZNT4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12623-1-AP

Synonyms

SLC30A4, Zinc transporter 4, ZnT 4, ZNT4, ZnT-4

---

Validation Data Gallery

• 

  WB analysis using 12623-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12623-1-AP (ZNT4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12623-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZNT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12623-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12623-1-AP (ZNT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12623-1-AP targets ZNT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNT4 fusion protein Ag3313
• Full Name: solute carrier family 30 (zinc transporter), member 4
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC026089
• Gene Symbol: SLC30A4
• Gene ID (NCBI): 7782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNT4, also known as SLC30A4, belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. ZNT4 transports Zn into the trans-Golgi apparatus for lactose synthesis, and across the apical cell membrane for efflux from MECs into milk. ZNT4 is expressed in numerous tissues and is enriched in the prostate. ZNT4 encodes a protein with a molecular weight of 47 kDa (PMID: 26538236).