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SLC30A8/ZNT8-Specific Polyclonal antibody

SLC30A8/ZNT8-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16169-1-AP

Synonyms

SLC30A8, SLC30A8/ZNT8-Specific, Zinc transporter 8, ZnT 8, ZNT8



Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Yellow catfish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name solute carrier family 30 (zinc transporter), member 8
Calculated molecular weight 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_173851
Gene symbol SLC30A8
Gene ID (NCBI) 169026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

Protocols

Product Specific Protocols
WB protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IHC protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
IF protocol for SLC30A8/ZNT8-Specific antibody 16169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.

Authors - Chengfeng Merriman
ratWB

J Biol Chem

PMID: 28130446

Authors - Qiong Huang
mouseWB

J Biol Chem

A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.

Authors - Chengfeng Merriman

J Biol Chem

Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.

Authors - Chengfeng Merriman
humanWB

J Endocrinol

Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death.

Authors - Maria Karsai
humanIHC

Sci Rep

A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.

Authors - Shogo Ohta