SLC30A8/ZNT8-Specific Polyclonal antibody

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  WB analysis using 16169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 16169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: 66198-1-Ig (Insulin antibody).

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  IF Staining of HepG2 using 16169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC30A8/ZNT8-Specific antibody (16169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SLC30A8/ZNT8-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 16169-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

SLC30A8, ZnT 8, Proton-coupled zinc antiporter SLC30A8, ZNT8, ZnT-8

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue, human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human pancreas tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

16169-1-AP targets SLC30A8/ZNT8-Specific in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, yellow catfish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 30 (zinc transporter), member 8
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_173851
• Gene Symbol: SLC30A8
• Gene ID (NCBI): 169026
• RRID: AB_10733889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWU4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Biol Chem; Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.; Authors - Chengfeng Merriman
rat	WB	J Biol Chem; PMID: 28130446; Authors - Qiong Huang
mouse	WB	J Biol Chem; A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.; Authors - Chengfeng Merriman
		J Biol Chem; Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.; Authors - Chengfeng Merriman
human	WB	J Endocrinol; Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death.; Authors - Maria Karsai
human	IHC	Sci Rep; A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.; Authors - Shogo Ohta