|Positive WB detected in||mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14471-1-AP targets VGAT in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Caenorhabditis elegans, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VGAT fusion protein Ag5843|
|Full Name||solute carrier family 32 (GABA vesicular transporter), member 1|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC053582|
|Gene ID (NCBI)||140679|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.
|Product Specific Protocols|
|WB protocol for VGAT antibody 14471-1-AP||Download protocol|
|IHC protocol for VGAT antibody 14471-1-AP||Download protocol|
|IF protocol for VGAT antibody 14471-1-AP||Download protocol|
|IP protocol for VGAT antibody 14471-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mandi (Verified Customer) (03-10-2020)
Did not stain the nerve terminals well. Signal was localized to the soma mainly.
Ute (Verified Customer) (03-03-2020)
blocking solution 5% skimmed milk in 1x TBS-T