SLC34A2 Polyclonal antibody

• 

  WB analysis of HEK-293 cells using 21295-1-AP

  HEK293 cells were subjected to SDS PAGE followed by western blot with 21295-1-AP (SLC34A2 Antibody) at dilution of 1:500.

• 

  IHC staining of human ovary cancer using 21295-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21295-1-AP (SLC34A2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SLC34A2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21295-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Na(+)/Pi cotransporter 2B, Na(+)-dependent phosphate cotransporter 2B, NaPi 2b, NAPI 3B, NAPI IIb

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

21295-1-AP targets SLC34A2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC34A2 fusion protein Ag15846
• Full Name: solute carrier family 34 (sodium phosphate), member 2
• Calculated Molecular Weight: 690 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC146666
• Gene Symbol: SLC34A2
• Gene ID (NCBI): 10568
• RRID: AB_2878835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95436
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC34A2, also known as NaPi-IIb, is a member of the solute carrier family 34 and encodes a type 2b sodium-dependent phosphate transporter. It is a multi-pass membrane protein composed of 690 amino acids. SLC34A2 is predominantly expressed in the lung, small intestine, and kidney. In the lung, it is specifically localized to the apical membrane of type II alveolar epithelial cells (ATII cells) and plays a crucial role during fetal lung development and embryonic development. It's reported that SLC34A2 is down-regulated in NSCLC tissues and cell lines. (PMID:26156586)

Publications

Species	Application	Title
human	WB	FEBS Open Bio; SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.; Authors - Jianlong Chen ; KD Validated