SLC38A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC38A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 12039-1-AP

Synonyms

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1

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Validation Data Gallery

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  WB analysis of Jurkat cells using 12039-1-AP

  WB result of SLC38A1 antibody (12039-1-AP, 1:500) with si-Control and si-SLC38A1 transfected Jurkat cells.

• 

  WB analysis of human brain using 12039-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12039-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12039-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12039-1-AP

  IP result of anti-SLC38A1 (IP:12039-1-AP, 4ug; Detection:12039-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 12039-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12039-1-AP (SLC38A1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, human kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12039-1-AP targets SLC38A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC38A1 fusion protein Ag2668
• Full Name: solute carrier family 38, member 1
• Calculated Molecular Weight: 487 aa, 54 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC010620
• Gene Symbol: SLC38A1
• Gene ID (NCBI): 81539
• RRID: AB_2190392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Publications

Species	Application	Title
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Environ Pollut; Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions; Authors - Jae Soon Kang
human	WB	J Cell Biol; TFEB controls retromer expression in response to nutrient availability.; Authors - Rachel Curnock
		PLoS One; Promotion of both proliferation and neuronal differentiation in pluripotent P19 cells with stable overexpression of the glutamine transporter slc38a1.; Authors - Ogura Masato M
human	IHC	J Oncol; High Expression Levels of SLC38A1 Are Correlated with Poor Prognosis and Defective Immune Infiltration in Hepatocellular Carcinoma.; Authors - Yun Liu