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SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12039-1-AP

Synonyms

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1



Tested Applications

Positive WB detected inJurkat cells, human brain tissue, human kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12039-1-AP targets SLC38A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC38A1 fusion protein Ag2668
Full Name solute carrier family 38, member 1
Calculated Molecular Weight 487 aa, 54 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC010620
Gene Symbol SLC38A1
Gene ID (NCBI) 81539
RRIDAB_2190392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Protocols

Product Specific Protocols
WB protocol for SLC38A1 antibody 12039-1-APDownload protocol
IHC protocol for SLC38A1 antibody 12039-1-APDownload protocol
IP protocol for SLC38A1 antibody 12039-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Authors - Dan Liu
humanIF

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
mouseWB

Environ Pollut

Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions

Authors - Jae Soon Kang
humanWB

J Cell Biol

TFEB controls retromer expression in response to nutrient availability.

Authors - Rachel Curnock

PLoS One

Promotion of both proliferation and neuronal differentiation in pluripotent P19 cells with stable overexpression of the glutamine transporter slc38a1.

Authors - Ogura Masato M
humanIHC

J Oncol

High Expression Levels of SLC38A1 Are Correlated with Poor Prognosis and Defective Immune Infiltration in Hepatocellular Carcinoma.

Authors - Yun Liu