SLC38A3 Polyclonal antibody, PBS Only

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• SLC38A3 Polyclonal antibody, PBS Only (14315-1-PBS)

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  WB analysis using 14315-1-AP (same clone as 14315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 14315-1-AP (same clone as 14315-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 14315-1-AP (same clone as 14315-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 14315-1-AP (same clone as 14315-1-PBS)

  IP result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 14315-1-AP (same clone as 14315-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SLC38A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14315-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

14315-1-PBS targets SLC38A3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5553

  Product name: Recombinant human SLC38A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-191 aa of BC042875

  Sequence: MEAPLQTEMVELVPNGKHSEGLLPVITPMAGNQRVEDPARSCMEGKSFLQKSPSKEPHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHLLLKSSGVVGIRAYEQLGYRAFGTPGKLAAALAITLQNIGAMSSYLYIIKSELPLVIQTFLNLEEKTSDWYMNGNY

• Full Name: solute carrier family 38, member 3
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC042875
• Gene Symbol: SLC38A3
• Gene ID (NCBI): 10991
• RRID: AB_2254879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99624
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.