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- KD/KO Validated
ZIP4 Polyclonal antibody
ZIP4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 20625-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Colo320 cells, human jejunum tissue |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 22 publications below |
| IHC | See 14 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 20625-1-AP is ZIP4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZIP4 fusion protein Ag14524 |
| Full Name | solute carrier family 39 (zinc transporter), member 4 |
| Calculated Molecular Weight | 647 aa, 68 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC062625 |
| Gene Symbol | ZIP4 |
| Gene ID (NCBI) | 55630 |
| RRID | AB_10700008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZIP4 is a zinc transporter encoded by the SLC39A4 gene, playing an important role in zinc homeostasis in mammalian cells. Recently, many studies have indicated that ZIP4 plays important roles in many kinds of carcinoma development and progression. ZIP4 may serve as a potential diagnostic and prognostic marker for gliomas and pancreatic cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIP4 antibody 20625-1-AP | Download protocol |
| IHC protocol for ZIP4 antibody 20625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells. | ||
Gastroenterology Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.
| ||
Clin Cancer Res ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.
| ||
Cancer Lett ZIP4 promotes non-small cell lung cancer metastasis by activating snail-N-cadherin signaling axis. | ||
Int J Mol Sci Zinc Favors Triple-Negative Breast Cancer's Microenvironment Modulation and Cell Plasticity | ||
Cancers (Basel) ZIP4 Is a Novel Cancer Stem Cell Marker in High-Grade Serous Ovarian Cancer. |
