Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, Calu-1 cells, L02 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19429-1-AP targets ZIP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13762 Product name: Recombinant human SLC39A7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 237-384 aa of BC000645 Sequence: VRHVKGGHGHSHGHGHAHSHTRGSHGHGRQERSTKEKQSSEEEEKETRGVQKRRGGSTVPKDGPVRPQNAEEEKRGLDLRVSGYLNLAADLAHNFTDGLAIGASFRGGRGLGILTTMTVLLHEVPHEVGDFAILVQSGCSKKQAMRLQ Predict reactive species |
| Full Name | solute carrier family 39 (zinc transporter), member 7 |
| Calculated Molecular Weight | 469 aa, 50 kDa |
| Observed Molecular Weight | 45-50 kDa, 56 kDa |
| GenBank Accession Number | BC000645 |
| Gene Symbol | ZIP7 |
| Gene ID (NCBI) | 7922 |
| RRID | AB_10643376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92504 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IHC protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IP protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| WB protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC39A7 (ZIP7) antibody (Cat# 19429-1-AP) has 24 citations published in journals including Nature Communications, Cell Death & Differentiation, Cell Death & Disease, Developmental Cell, Autophagy, Metallomics, and PLoS ONE. Associated research fields encompass zinc homeostasis, ferroptosis, neurodegeneration, cancer biology (breast, colorectal, hepatocellular carcinoma), mitochondrial dynamics, endoplasmic reticulum stress, immune modulation, and pancreatic β-cell function.
| Species | Application | Title |
|---|---|---|
Autophagy The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking | ||
Nat Commun The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes. | ||
Cell Death Differ Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. | ||
Sci Total Environ ACSL4-mediated ZIP7-VDAC3 interaction regulates endoplasmic reticulum-mitochondria iron transfer in hepatocytes under PFOS exposure |
