SLC45A2 Polyclonal antibody

SLC45A2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10453-1-AP

Synonyms

1A1, AIM1, MATP, Melanoma antigen AIM1, Protein AIM 1, SHEP5, SLC45A2

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Validation Data Gallery

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  IHC staining of mouse colon using 10453-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10453-1-AP (SLC45A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10453-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10453-1-AP (SLC45A2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 10453-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10453-1-AP (SLC45A2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10453-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10453-1-AP (SLC45A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse colon tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10453-1-AP targets SLC45A2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC45A2 fusion protein Ag0699
• Full Name: solute carrier family 45, member 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC003597
• Gene Symbol: SLC45A2
• Gene ID (NCBI): 51151
• RRID: AB_2191075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC45A2, also named as AIM1 and MATP, belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family. It is a melanocyte differentiation antigen. SLC45A2 may transport substances required for melanin biosynthesis. (PMID:17768386,21677667)Mutation of SLC45A2 cause oculocutaneous albinism type 4 (OCA4) which is disorder of pigmentation characterized by reduced biosynthesis of melanin in the skin, hair and eyes.

Publications

Species	Application	Title
human	WB,IF	J Invest Dermatol; Homozygosity mapping and whole-exome sequencing to detect SLC45A2 and G6PC3 mutations in a single patient with oculocutaneous albinism and neutropenia.; Authors - Cullinane Andrew R AR
human	IHC	Front Oncol; New Prognostic Biomarkers and Drug Targets for Skin Cutaneous Melanoma via Comprehensive Bioinformatic Analysis and Validation.; Authors - Sitong Zhou