SLC4A10 Polyclonal antibody

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  WB analysis using 27197-1-AP

  Boiled and 37°C incubated mouse brain tissues were subjected to SDS PAGE followed by western blot with 27197-1-AP (SLC4A10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27197-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27197-1-AP (SLC4A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27197-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27197-1-AP (SLC4A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC4A10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27197-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

NBCn2, NCBE, SLC4A10

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

27197-1-AP targets SLC4A10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26102

  Product name: Recombinant human SLC4A10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-94 aa of NM_001178015

  Sequence: TRNDEEAVVDRGGTRSILKTHFEKEDLEGHRTLFIGVHVPLGGRKSHRRHRHRGHKHRKRDRERDSGLEDGRESPSFDT

• Full Name: solute carrier family 4, sodium bicarbonate transporter, member 10
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001178015
• Gene Symbol: SLC4A10
• Gene ID (NCBI): 57282
• RRID: AB_3085934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6U841
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Mol Clin Oncol; Single-cell analysis reveals distinct immune characteristics of hepatocellular carcinoma in HBV-positive vs. HBV-negative cases; Authors - Ke Liu