SLC6A11 Polyclonal antibody

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  WB analysis of mouse cerebellum using 13920-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13920-1-AP (SLC6A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13920-1-AP (SLC6A11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13920-1-AP (SLC6A11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC6A11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13920-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

GABT3, GAT 3, GAT3, SLC6A11

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13920-1-AP targets SLC6A11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4927

  Product name: Recombinant human SLC6A11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-208 aa of BC036083

  Sequence: MTAEKALPLGNGKAAEEARESEAPGGGCSSGGAAPARHPRVKRDKAVHERGHWNNKVEFVLSVAGEIIGLGNVWRFPYLCYKNGGGAFLIPYVVFFICCGIPVFFLETALGQFTSEGGITCWRKVCPLFEGIGYATQVIEAHLNVYYIIILAWAIFYLSNCFTTELPWATCGHEWNTENCVEFQKLNVSNYSHVSLQNATSPVMEFWE

• Full Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 11
• Calculated Molecular Weight: 71 and 23 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC036083
• Gene Symbol: SLC6A11
• Gene ID (NCBI): 6538
• RRID: AB_2877988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	J Neurochem; Cooperative and competitive regulation of the astrocytic transcriptome by neurons and endothelial cells: Impact on astrocyte maturation; Authors - Zila Martinez-Lozada
mouse	WB	Ecotoxicol Environ Saf; 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway; Authors - Yuchen Qin
mouse	WB	Neuropharmacology; A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine; Authors - Xiao-Hui Tang