SLC7A1/CAT-1 Polyclonal antibody

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• KD/KO Validated

SLC7A1/CAT-1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14195-1-AP Copied!

Publications (42)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC (Intra), CoIP, ELISA

Synonyms

CAT-1, SLC7A1, CAT1, ERR

• 

  WB analysis of rat brain/liver tissue using 14195-1-AP

  WB result of 14195-1-AP from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 14195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, Jurkat cells, rat brain/liver tissue, rat liver tissue
• Positive IHC detected in: human ovary tumor tissue, human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14195-1-AP targets SLC7A1/CAT-1 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5398

  Product name: Recombinant human SLC7A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 312-629 aa of BC063303

  Sequence: MPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK

• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC063303
• Gene Symbol: CAT-1
• Gene ID (NCBI): 6541
• RRID: AB_2190723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30825
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC7A1, also known as CAT-1, belongs to the SLC7 subfamily of the amino acid-polyamine-organocation (APC) superfamily. SLC7A1 is a high-affinity, low capacity permease involved in mediating the uptake of L-arginine, L-lysine, L-ornithine, and L-histidine from the extracellular environment by cells.

Publications

What published studies show

Anti-SLC7A1 (CAT1) antibody (Cat# 14195-1-AP) has 42 citations published in journals including Nature, Cell, Nature Communications, Nature Cell Biology, EMBO Journal, Cancer Research, and Biomaterials. Research fields span amino acid metabolism, ferroptosis, oncology (colorectal, ovarian, pancreatic, osteosarcoma, hepatocellular), immunotherapy resistance, T cell biology, liver fibrosis, hypertension, cachexia, and neurodegeneration.

Automatically generated from the 42 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Spermine is an endogenous iron chelator that inhibits ferroptosis; Authors - Man Li ; KO Validated
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
human	WB	Nat Cell Biol; The lncRNA DAMER selectively guides m6A-dependent regulation of ATF4 and asparagine metabolism under nutrient stress in cancer; Authors - Tianyu Tao
human	IHC	Cancer Res; Arginine Deprivation Induces Quiescence and Confers Vulnerability to Ferroptosis in Colorectal Cancer; Authors - Yanyun Lin
		Nat Commun; Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer; Authors - Hanshen Yang
mouse,human	WB	Biomaterials; Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy; Authors - Xuemei Yao ; KD Validated